Journal: Diagnostic Microbiology and Infectious Disease

Article Title: Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens

doi: 10.1016/j.diagmicrobio.2018.04.006

Figure Lengend Snippet: Sensitivity of the multiplex RT-SIBA Influenza assay for the detection of influenza viruses. (a) Influenza A H1N1 virus. (b) Influenza A H3N2 virus. (c) Influenza B virus. (d) Melting curve profiles of influenza viruses (200 copies of influenza A H1N1, influenza A H3N2, and influenza B RNA).

Article Snippet: Purified and quantified viral particles from influenza A H1N1 (American Type Culture Collection (ATCC) VR-95PQ), influenza A H3N2 (ATCC VR-544PQ), and influenza B (ATCC VR-1804PQ) were obtained from ATCC (LGC Standards, Germany).

Techniques: Multiplex Assay, Virus

Journal: Diagnostic Microbiology and Infectious Disease

Article Title: Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens

doi: 10.1016/j.diagmicrobio.2018.04.006

Figure Lengend Snippet: Limit of detection of the multiplex RT-SIBA Influenza assay.

Article Snippet: Purified and quantified viral particles from influenza A H1N1 (American Type Culture Collection (ATCC) VR-95PQ), influenza A H3N2 (ATCC VR-544PQ), and influenza B (ATCC VR-1804PQ) were obtained from ATCC (LGC Standards, Germany).

Techniques: Multiplex Assay, Virus

Journal: Viruses

Article Title: An Examination of the Long-Term Neurodevelopmental Impact of Prenatal Zika Virus Infection in a Rat Model Using a High Resolution, Longitudinal MRI Approach

doi: 10.3390/v13061123

Figure Lengend Snippet: Absolute whole-brain volumes in postnatal day 2 neonates across all three groups stratified by sex using both automated and manual methods of segmentation ( n = 8–10 per sex per group). ( A ) Automated segmentation revealed a main effect of inoculation ( # p < 0.05). Specifically, whole-brain volume was significantly smaller in iZIKV pups when compared to both mock control and ZIKV pups (post-hoc, # p < 0.05). Analysis also revealed a main effect of sex (* p < 0.05); P2 females across all groups had significantly smaller whole-brain volumes than their male counterparts. No interaction between inoculation and sex was detected. ( B ) Manual segmentation also revealed a main effect of inoculation ( # p < 0.05). iZIKV pups again displayed significantly reduced whole-brain volumes when compared with all other groups; however, ZIKV pups also displayed significantly reduced whole-brain volumes when compared to mock controls (post-hoc, # p < 0.05). There was no main effect of sex or interaction of inoculation × sex. Bars represent the mean ± SD and data points represent individual subjects. Data were analyzed using a two-way ANOVA with inoculation ( # p < 0.05) and sex (* p < 0.05) as factors, followed by Tukey’s test.

Article Snippet: Zika virus stocks (strain PRVABC059, Puerto Rico, Human, December 2015 from ATCC) were propagated in Vero cells and serially passaged to yield high-titer virus stocks.

Techniques: Control

Journal: Viruses

Article Title: An Examination of the Long-Term Neurodevelopmental Impact of Prenatal Zika Virus Infection in a Rat Model Using a High Resolution, Longitudinal MRI Approach

doi: 10.3390/v13061123

Figure Lengend Snippet: Absolute whole-brain volumes in postnatal day 16 juveniles across all three groups stratified by sex using both automated and manual methods of segmentation ( n = 9–10 per sex per group). ( A ) Automated segmentation revealed a main effect of inoculation ( # p < 0.05). Specifically, whole-brain volume was significantly larger in ZIKV juveniles when compared to the mock control group (post-hoc, # p < 0.05). ( B ) Manual segmentation also revealed a main effect of inoculation ( # p < 0.05). Both iZIKV and ZIKV juveniles displayed significantly reduced whole-brain volumes when compared to mock control juveniles (post-hoc, # p < 0.05 ). Analyses also revealed a main effect of sex for both segmentation methods (* p < 0.05). More specifically, P16 females across all groups had significantly smaller whole-brain volumes than their male counterparts. There was no interaction of inoculation × sex within either method of segmentation. Bars represent the mean ± SD and data points represent individual subjects. Data were analyzed using a two-way ANOVA with inoculation ( # p < 0.05) and sex (* p < 0.05) as factors, followed by Tukey’s test.

Article Snippet: Zika virus stocks (strain PRVABC059, Puerto Rico, Human, December 2015 from ATCC) were propagated in Vero cells and serially passaged to yield high-titer virus stocks.

Techniques: Control

Journal: Viruses

Article Title: An Examination of the Long-Term Neurodevelopmental Impact of Prenatal Zika Virus Infection in a Rat Model Using a High Resolution, Longitudinal MRI Approach

doi: 10.3390/v13061123

Figure Lengend Snippet: Absolute whole-brain volumes in postnatal day 24 juveniles across all three groups stratified by sex using both methods of segmentation ( n = 7–10 per sex per group). ( A ) Automated segmentation revealed no main effect of inoculation. ( B ) Manual segmentation revealed a main effect of inoculation ( # p < 0.05). ZIKV juveniles displayed significantly reduced whole-brain volumes when compared to mock control juveniles (post-hoc, # p < 0.05). Analyses also revealed a main effect of sex for both segmentation methods (* p < 0.05). More specifically, P24 females across all groups had significantly smaller whole-brain volumes than their male counterparts. There was no interaction of inoculation × sex within either method of segmentation. Bars represent the mean ± SD and data points represent individual subjects. Data were analyzed using a two-way ANOVA with inoculation ( # p < 0.05) and sex (* p < 0.05) as factors, followed by Tukey’s test.

Article Snippet: Zika virus stocks (strain PRVABC059, Puerto Rico, Human, December 2015 from ATCC) were propagated in Vero cells and serially passaged to yield high-titer virus stocks.

Techniques: Control

Journal: Viruses

Article Title: An Examination of the Long-Term Neurodevelopmental Impact of Prenatal Zika Virus Infection in a Rat Model Using a High Resolution, Longitudinal MRI Approach

doi: 10.3390/v13061123

Figure Lengend Snippet: ( A ) Automated segmentation of brain regions at postnatal day 2 revealed a significant effect of inoculation. Analysis revealed ZIKV-inoculated neonates had a smaller isocortex when compared to mock-inoculated neonates (post-hoc, # p < 0.05). Furthermore, analysis revealed a significant effect of sex in both the isocortex and cerebellum ( * p < 0.05). Analysis detected no interaction of inoculation × sex. ( B ) Manual segmentation of brain regions at postnatal day 2 revealed a significant effect of inoculation. Within the isocortex, iZIKV-inoculated neonates displayed smaller regional volumes when compared to both mock and ZIKV counterparts (post-hoc, # p < 0.05). Analysis also revealed iZIKV neonates had smaller cerebellums in comparison to the mock controls (post-hoc, # p < 0.05). Analysis detected no significant effect of sex or interaction of inoculation × sex. Bars represent the mean ± SD and data points represent individual subjects ( n = 8–10 per sex per group). Data were analyzed using a two-way ANOVA with inoculation ( # p < 0.05) and sex ( * p < 0.05) as factors, followed by Tukey’s test.

Article Snippet: Zika virus stocks (strain PRVABC059, Puerto Rico, Human, December 2015 from ATCC) were propagated in Vero cells and serially passaged to yield high-titer virus stocks.

Techniques: Comparison

Journal: Viruses

Article Title: An Examination of the Long-Term Neurodevelopmental Impact of Prenatal Zika Virus Infection in a Rat Model Using a High Resolution, Longitudinal MRI Approach

doi: 10.3390/v13061123

Figure Lengend Snippet: ( A ) Automated segmentation of brain regions at postnatal day 16 revealed a significant effect of inoculation. Within the hippocampus region, ZIKV-inoculated juveniles displayed smaller regional volumes when compared to both mock and iZIKV counterparts (post-hoc, # p < 0.05). Within the isocortex and cerebellum, both iZIKV-inoculated and ZIKV-inoculated juveniles displayed smaller regional volumes when compared to mock controls (post-hoc, # p < 0.05). Analysis detected no significant effect of sex or interaction of inoculation × sex. ( B ) Manual segmentation of brain regions at postnatal day 16 revealed a significant effect of inoculation. Within the hippocampus region, ZIKV-inoculated juveniles displayed larger regional volumes when compared to both mock and iZIKV counterparts (post-hoc, # p < 0.05). Within the isocortex, iZIKV-inoculated juveniles displayed smaller regional volumes when compared to both mock and ZIKV counterparts (post-hoc, # p < 0.05). Analysis detected no significant effect of sex or interaction of inoculation × sex. Bars represent the mean ± SD and data points represent individual subjects ( n = 9–10 per sex per group). Data were analyzed using a two-way ANOVA with inoculation ( # p < 0.05) and sex as factors, followed by Tukey’s test.

Article Snippet: Zika virus stocks (strain PRVABC059, Puerto Rico, Human, December 2015 from ATCC) were propagated in Vero cells and serially passaged to yield high-titer virus stocks.

Techniques:

Journal: Viruses

Article Title: An Examination of the Long-Term Neurodevelopmental Impact of Prenatal Zika Virus Infection in a Rat Model Using a High Resolution, Longitudinal MRI Approach

doi: 10.3390/v13061123

Figure Lengend Snippet: ( A ) Automated segmentation of brain regions for postnatal day 24 revealed a main effect of sex in all regions analyzed ( * p < 0.05). Furthermore, analysis identified no significant effect of inoculation or interaction of inoculation × sex. ( B ) Manual segmentation of brain regions at postnatal day 24 revealed a significant effect of inoculation. Within the cerebellum region, ZIKV-inoculated juveniles displayed smaller regional volumes when compared to the iZIKV-inoculated juveniles (post-hoc, # p < 0.05). Analysis detected no significant effect of sex or interaction of inoculation × sex. Bars represent the mean ± SD and data points represent individual subjects ( n = 7–10 per sex per group). Data were analyzed using a two-way ANOVA with inoculation ( # p < 0.05) and sex ( * p < 0.05) as factors, followed by Tukey’s test.

Article Snippet: Zika virus stocks (strain PRVABC059, Puerto Rico, Human, December 2015 from ATCC) were propagated in Vero cells and serially passaged to yield high-titer virus stocks.

Techniques:

Journal: Viruses

Article Title: An Examination of the Long-Term Neurodevelopmental Impact of Prenatal Zika Virus Infection in a Rat Model Using a High Resolution, Longitudinal MRI Approach

doi: 10.3390/v13061123

Figure Lengend Snippet: ( A ) Automated segmentation of brain regions at postnatal day 60 revealed a significant effect of inoculation. Within the hippocampus region, ZIKV-inoculated juveniles displayed smaller regional volumes when compared to the iZIKV-inoculated juveniles (post-hoc, # p < 0.05). Moreover, analysis also revealed a main effect of sex within the hippocampal region ( * p < 0.05). Analysis detected no interaction of inoculation × sex. ( B ) Analysis of manually segmented brain regions for postnatal day 60 revealed no significant effect of inoculation, sex, or interaction of inoculation × sex. Bars represent the mean ± SD and data points represent individual subjects ( n = 7–10 per sex per group). Data were analyzed using a two-way ANOVA with inoculation ( # p < 0.05) and sex ( * p < 0.05) as factors, followed by Tukey’s test.

Article Snippet: Zika virus stocks (strain PRVABC059, Puerto Rico, Human, December 2015 from ATCC) were propagated in Vero cells and serially passaged to yield high-titer virus stocks.

Techniques:

Journal: Viruses

Article Title: An Examination of the Long-Term Neurodevelopmental Impact of Prenatal Zika Virus Infection in a Rat Model Using a High Resolution, Longitudinal MRI Approach

doi: 10.3390/v13061123

Figure Lengend Snippet: Analysis of manually segmented hippocampal subregions for postnatal day 2 ( n = 17–19 per group) ( A ), postnatal day 16 ( n = 18–20 per group) ( B ), postnatal day 24 ( n = 15–20 per group) ( C ), and postnatal day 60 ( n = 16–19 per group) ( D ). At P2, volumetric analysis of hippocampal subregions revealed a significant effect of inoculation within the CA3 of the hippocampus. Specifically, the ZIKV-inoculated pups displayed a significant volume reduction in the CA3 subregion when compared to mock counterparts (post-hoc, * p < 0.05). At P16, analysis revealed a significant effect of inoculation within the CA1 of the hippocampus and dentate gyrus. Specifically, ZIKV-inoculated juveniles displayed significantly larger volumes in both the CA1 subregion and dentate gyrus when compared to mock controls (post-hoc, * p < 0.05 ) . Across all groups, bars represent the mean ± SD in each subregion of the hippocampus while data points represent each individual rat.

Article Snippet: Zika virus stocks (strain PRVABC059, Puerto Rico, Human, December 2015 from ATCC) were propagated in Vero cells and serially passaged to yield high-titer virus stocks.

Techniques: